Cap N’ Stem

▶

▶ 571 ❤ 0 💬 0

Mechanical Dedikaryotization: what a mouthful...but if you know...you know. The power of the monokaryon is something not to be underestimated and overlooked. The simplicity of a protocol written up by @dedikariotizationdaddy and derived from Hermilo Leal-Lara’s work on “monokaryotization” from the early 1980’s is the basis for this week’s episode. Outside of Julian’s work creating a monokaryotic library from fungal phenotypes, no other company or institution we are aware of has presented an interest in replicating the work of splitting all of their dikaryons to increase the amount of cultivators taking “crosses” into their own labs. Over this winter @mainecapnstemco will be developing a library of cryogenically preserved “mons” to be used in future breeding programs. These mons will be available through our website as we confirm the lack of clamps and start the lines and loops of cryo-preservation. As for today’s Deep Dive, we took extra care and time to delve into the realities of mechanical-dedi-work. While there are other methods for isolating the two “mons” that make up a “di”, we wanted to initially explore this simple methodology with our dedicated MycoWizards members to rekindle an interest in lab work beyond the expansions for fruit body production. So dust off your microscopes, bust out your blenders and let's get down to it! #mycowizards #mycology #mycowizardspodcast #ommushrooms #lipatek #shroomery #medicinalmushrooms #woodmeat #shroomsofdoom #mainecapnstem #capnstem #mushrooms #growmoremushrooms #mycelium #mushrooms #mushroom #substrate #farming #coco #fenrir #capnstem #walkthrough #growmushrooms #howto #growyourown #sustainable #farm #equipment #fabrication #diy

▶

▶ 308 ❤ 0 💬 0

Erik and Gregor take a break from the flow hood buzz to have a wander in the great outdoors. It wasn't long before they made the acquaintance of some hens, chicken and shrimp out in the wilderness. Being the hospitable gentlemen that they are, the boys brought them back home to the lab and chopped them up. Its wild mushroom capturing this week, get the net. Watch and listen as our one and only Gregor breaks out some plates and a microscope to tell the tale of the clones that lived, died and tried. On the dishes and in our hearts. Along the way of cleaning culture after culture we encountered mold and bacteria of multiple varieties until we finally isolated some healthy mycelium, and successfully cloned our wild. Come hang out in the woods with us. only at MycoWizards.com USE CODE MYCOTOK AT CHECKOUT FOR 30 days FREE #wildmushrooms #cloning #fungi #naturewalk #chickenofthewoods #henofthewoods #shrimpofthewoods #mushroom #mushrooms #mycelium #mycology #culturebank #mycowizards #maine #lewistonmaine #mushroomforaging #foraging #newepisode #deepdive #howto

▶

▶ 413 ❤ 0 💬 0

The original framework for today's episode was inspired by the "bioreactor" lids from @myco_op ... The original question we were looking to answer: Does the forced introduction of sterile air into a forming liquid culture produce faster colonization rates and higher yields over substrates inoculated with a standard liquid culture? While the relatively new adoption of aerated LC is in its infancy industry wide right now, the idea of conditioning a mycelium in an aqueous solution is something that everyone should consider. What are the values above the risks associated with liquid culture production in general? While we answer our original question and many more in this week's MycoWizards Deep Dive, a million more questions still stand and many observations remain unexplored. So join us in uncovering some of the secrets of the Aerated Liquid Culture at mycowizards.com and if you feel the deep desire to jump in on the work yourself, use the code MYCOWIZARD over at https://myco-op.online to get yourself 20% off bioreactor lids! We are currently slow-releasing 20ML Aerated Liquid Cultures at capnstem.com ... so get some today and test them against what else is out there and report back! #mycowizards #mycology #mycowizardspodcast #ommushrooms #lipatek #shroomery #medicinalmushrooms #woodmeat #shroomsofdoom #mainecapnstem #capnstem #mushrooms #growmoremushrooms #mycelium #mushrooms #mushroom #substrate #farming #coco #fenrir #capnstem #walkthrough #growmushrooms #howto #growyourown #sustainable #farm #equipment #fabrication #diy